| Autor: |
E D, Segal, L S, Tompkins |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
BioTechniques. 14(2) |
| ISSN: |
0736-6205 |
| Popis: |
A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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