| Autor: |
Andrey D, Prjibelski, Alla, Mikheenko, Anoushka, Joglekar, Alexander, Smetanin, Julien, Jarroux, Alla L, Lapidus, Hagen U, Tilgner |
| Rok vydání: |
2022 |
| Zdroj: |
Nature biotechnology. |
| ISSN: |
1546-1696 |
| Popis: |
Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant-a computational tool using intron graphs that accurately reconstructs transcripts both with and without reference genome annotation. For novel transcript discovery, IsoQuant reduces the false-positive rate fivefold and 2.5-fold for Oxford Nanopore reference-based or reference-free mode, respectively. IsoQuant also improves performance for Pacific Biosciences data. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink