Single cell gene expression analysis of pluripotent stem cells

Autor:	Ronald V, Abruzzese, Richard A, Fekete
Rok vydání:	2013
Předmět:	Pluripotent Stem Cells Reverse Transcriptase Polymerase Chain Reaction Animals Gene Expression Humans Single-Cell Analysis Real-Time Polymerase Chain Reaction Transcriptome Cells Cultured
Zdroj:	Methods in molecular biology (Clifton, N.J.). 997
ISSN:	1940-6029
Popis:	Analyzing gene expression profiles from cells en masse provides an average profile for the population which may obscure differences in individual cells. Using an optimized workflow for qRT-PCR, gene expression profiles of undifferentiated pluripotent stem cells reveal distinct gene expression profiles for individual cells, and a large expression level range of almost every gene. Importantly, this technique allows for the identification and characterization of small subpopulations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::16751b67e18e4e38eb3cdf7dbf673971 https://pubmed.ncbi.nlm.nih.gov/23546759 Zobrazit plný text záznamu