Disruption of six Saccharomyces cerevisiae novel genes and phenotypic analysis of the deletants

Autor:	S, Zúñiga, J, Boskovic, A, Jiménez, J P, Ballesta, M, Remacha
Rok vydání:	1999
Předmět:	Fungal Proteins Open Reading Frames Phenotype Metals Genes Fungal Genetic Complementation Test Oligonucleotides Saccharomyces cerevisiae Cloning Molecular Physical Chromosome Mapping Polymerase Chain Reaction Gene Deletion Plasmids
Zdroj:	Yeast (Chichester, England). 15(10B)
ISSN:	0749-503X
Popis:	As a part of the EUROFAN programme, six open reading frames from Saccharomyces cerevisiae (YNL083w, YNL086w, YNL087w, YNL097c, YDL100c and YOR086c) were disrupted in two genetic backgrounds, FY1679 and W303. Individual deletions in diploid strains and tetrad analysis of heterozygous deletants revealed that none of them is essential. Basic phenotypic analysis did not reveal any significant difference between the parental and mutant strains. Although YNL087w and YOR086c are 55% identical, the double disruptant also behaves the same as the parental cells. Ydl100p seems to be involved in metal detoxification, the phenotype of the null mutants being enhanced when the assays are performed at 37 degrees C.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::15c0361da73ff0df8eb2a8804b439083 https://pubmed.ncbi.nlm.nih.gov/10407274 Zobrazit plný text záznamu Plný text