Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
| Autor: | Kaths, J. Moritz, Spetzler, Vinzent N., Goldaracena, Nicolas, Echeverri, Juan, Louis, Kristine S., Foltys, Daniel B., Strempel, Mari, Yip, Paul, John, Rohan, Mucsi, Istvan, Ghanekar, Anand, Bagli, Darius, Robinson, Lisa, Selzner, Markus |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
extended criteria donor (ECD) Swine delayed graft function (DGF) Organ Preservation hypothermic machine perfusion (HMP) Kidney Kidney Transplantation Perfusion Issue 101 organ shortage normothermic ex vivo kidney perfusion (NEVKP) standard criteria donor (SCD) Models Animal Medicine primary non function (PNF) Animals marginal graft donation after circulatory death (DCD) cold storage (CS) |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | Kidney transplantation has become a well-established treatment option for patients with end-stage renal failure. The persisting organ shortage remains a serious problem. Therefore, the acceptance criteria for organ donors have been extended leading to the usage of marginal kidney grafts. These marginal organs tolerate cold storage poorly resulting in increased preservation injury and higher rates of delayed graft function. To overcome the limitations of cold storage, extensive research is focused on alternative normothermic preservation methods. Ex vivo normothermic organ perfusion is an innovative preservation technique. The first experimental and clinical trials for ex vivo lung, liver, and kidney perfusions demonstrated favorable outcomes. In addition to the reduction of cold ischemic injury, the method of normothermic kidney storage offers the opportunity for organ assessment and repair. This manuscript provides information about kidney retrieval, organ preservation techniques, and isolated ex vivo normothermic kidney perfusion (NEVKP) in a porcine model. Surgical techniques, set up for the perfusion solution and the circuit, potential assessment options, and representative results are demonstrated. |
| Databáze: | OpenAIRE |
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