Hydroxytyrosol, a dietary phenolic compound forestalls the toxic effects of methylmercury-induced toxicity in IMR-32 human neuroblastoma cells
| Autor: | Vishnu, Mohan, Shubhankar, Das, Satish B S, Rao |
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| Rok vydání: | 2014 |
| Předmět: |
Caspase 3
Cell Survival NF-E2-Related Factor 2 Superoxide Dismutase Cytochromes c DNA Fragmentation Methylmercury Compounds Phenylethyl Alcohol Catalase Glutathione Neuroblastoma Oxidative Stress Microscopy Fluorescence Cell Line Tumor Humans Comet Assay Tumor Suppressor Protein p53 bcl-2-Associated X Protein |
| Zdroj: | Environmental toxicology. 31(10) |
| ISSN: | 1522-7278 |
| Popis: | This study demonstrates the protective potential of hydroxytyrosol (HT), an olive oil phenol, against methylmercury (MeHg)-induced neurotoxicity using IMR-32 human neuroblastoma cell line. HT inhibited MeHg-induced cytotoxicity and genotoxicity as confirmed by MTT, micronucleus, and comet assays. Cells preconditioned with HT showed reduction of MeHg-induced cellular oxidative stress along with the maintenance of glutathione, superoxide dismutase, glutathione-S-tranferase, and catalase. Fluorescence microscopy and DNA ladder assays indicated the inhibitory effect of HT against MeHg-induced apoptosis, which was further established by Western blotting. An effective concentration of 5 µM HT caused downregulation of p53, bax, cytochrome c, and caspase 3 and upregulation of prosurvival proteins including nuclear factor erythroid 2-related factor 2 (Nrf2) and metallothionein. This work indicates the cytoprotective potential of HT against MeHg-induced toxicity primarily by the lowering of oxidative stress, which may be endorsed to its antigenotoxic and antiapoptotic potential, in addition to its free radical scavenging ability. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1264-1275, 2016. |
| Databáze: | OpenAIRE |
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