Infectious in vitro transcripts from a cDNA clone of a Japanese gentian isolate of Sikte waterborne virus, which shows host-specific low-temperature-dependent replication

Autor: Koki, Fujisaki, Chika, Tateda, Yoshiko, Abe, John Jewish A, Dominguez, Mari, Iwai, Kazue, Obara, Taiki, Nakamura, Yasuya, Iwadate, Masanori, Kaido, Kazuyuki, Mise
Rok vydání: 2021
Předmět:
Zdroj: Archives of virology. 166(7)
ISSN: 1432-8798
Popis: Tombusviruses have been identified in several crops, including gentian virus A (GeVA) in Japanese gentian. In this study, we isolated another tombusvirus, Sikte waterborne virus strain C1 (SWBV-C1), from Japanese gentian. Although SWBV-C1 and GeVA are not closely related, SWBV-C1, like GeVA, showed host-specific low-temperature-dependent replication in gentian and arabidopsis. The use of in vitro transcripts from full-length cDNA clones of SWBV-C1 genomic RNA as inocula confirmed these properties, indicating that the identified genomic RNA sequences encode viral factors responsible for the characteristic features of SWBV-C1.
Databáze: OpenAIRE