Advances in the analysis of conformational transitions in peptides using differential scanning calorimetry

Autor: Werner W, Streicher, George I, Makhatadze
Rok vydání: 2006
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 350
ISSN: 1064-3745
Popis: Differential scanning calorimetry can measure the heat capacity of a protein/peptide solution over a range of temperatures at constant pressure, which is used to determine the enthalpy function of the system. There are several experimental factors that can have a significant impact on the determined enthalpy and subsequent derived thermodynamic parameters. These factors are discussed in terms of sample and instrument preparation, as well as data collection and analysis.
Databáze: OpenAIRE