Human anti-DNA secretory immunglobulins A possess endonuclease activity and they are able to cause the destruction of nuclear chromatin in vitro

Autor:	YuYa, Kit, E E, Mitrofanova, O E, Shestova, E V, Kuligina, I V, Romannikova, V A, Richter
Rok vydání:	2001
Předmět:	Cell Nucleus Milk Human Antibodies Antinuclear Humans Electrophoresis Polyacrylamide Gel DNA In Vitro Techniques Chromatography Ion Exchange Protein Kinases Chromatin Immunoglobulin A
Zdroj:	Ukrains'kyi biokhimichnyi zhurnal (1999 ). 72(3)
Popis:	sIgA possessing ability to hydrolyse plasmid DNA to linear forms was purified from human milk by sequential chromatography on protein A-sepharose, DEAE-Fractogel and DNA-cellulose. It was discovered that incubation of sIgA with nuclei of porcine embryo kidney cells permeabilized by Triton X-100 causes formation of electrophoretically mobile forms of nuclear nucleic acids and inhibition of phosphorylation of nuclear proteins. We suppose that sIgA possessing affinity to DNA and endonuclease activity can cause degradation of cell nuclear chromatin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1341afefa782e7138eca1c35c78a8271 https://pubmed.ncbi.nlm.nih.gov/11200479 Zobrazit plný text záznamu