Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

Autor:	G W, Black, C M, Lyons, E, Williams, J, Colby, M, Kehoe, C, O'Reilly
Rok vydání:	1990
Předmět:	Genomic Library Multienzyme Complexes Pseudomonas Blotting Western Restriction Mapping Escherichia coli Gene Expression Cloning Molecular Aldehyde Oxidoreductases
Zdroj:	FEMS microbiology letters. 58(3)
ISSN:	0378-1097
Popis:	Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::131f4c082c19425fedee4e002ecb6eac https://pubmed.ncbi.nlm.nih.gov/2227359 Zobrazit plný text záznamu Plný text