| Autor: |
Simona, Gribaudo, Luc, Bousset, Josquin, Courte, Ronald, Melki, Jean-Michel, Peyrin, Anselme L, Perrier |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2551 |
| ISSN: |
1940-6029 |
| Popis: |
Aggregated alpha-synuclein (α-Syn) in neurons is a hallmark of Parkinson's disease (PD) and other synucleinopathies. Recent advances (1) in the production and purification of synthetic assemblies of α-Syn, (2) in the design and production of microfluidic devices allowing the construction of oriented and compartmentalized neuronal network on a chip, and (3) in the differentiation of human pluripotent stem cells (hPSCs) into specific neuronal subtypes now allow the study of cellular and molecular determinants of the prion-like properties of α-Syn in vitro. Here, we described the methods we used to reconstruct a cortico-cortical human neuronal network in microfluidic devices and how to take advantage of this cellular model to characterize (1) the prion-like properties of different α-Syn strains and (2) the neuronal dysfunctions and the alterations associated with the exposure to α-Syn strains or the nucleation of endogenous α-Syn protein in vitro. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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