| Popis: |
Testicular oxidative stress, endocrine disruption and abnormal spermatogenesis in rats exposed to high doses ofphosphodiesterase-5 inhibitors (PDE5i) and opioids, with poor reversibility following withdrawal of treatment had beenreported. In this study, we examined the histopathological effects of high doses of sildenafil, tadalafil, tramadol andsildenafil+tramadol on the testes and epididymis of rats. Seventy male rats (180 - 200 g b.w) were assigned to one of fivegroups (n = 14), namely; A: control (0.2 mL normal saline), B: sildenafil (1 mg/100g b.w), C: tadalafil (1 mg/100g b.w), D:tramadol (2 mg/100g b.w) and E: sildenafil+tramadol group (dose as in groups B and D). The drugs were administered orallyfor 8 weeks. Seven rats were sacrificed per group while the remaining 7/group continued for 8 weeks without treatment.Histopathological examination was carried out at the end of both phases. After 8 weeks of treatment, mean Johnsen'stesticular biopsy score (MJTBS) and Leydig cell count decreased significantly (p=0.001) in all treated groups compared withthe control. The MJTBS and Leydig cell count decreased significantly in tramadol (p = 0.05) and sildenafil+tramadol (p0.01)groups compared with tadalafil group. After recovery, MJTBS and Leydig cell count were significantly (p0.05) lower in all the groups compared with the control. Histology of the testes of rats in groups B - E showed reduced germ cell andspermatozoa population in the seminiferous tubules after 8 weeks treatment. Additionally, their epididymis showed decreasedspermatozoa density. There was no complete reversibility of histopathological alterations following withdrawal of treatment.High doses of sildenafil, tadalafil, tramadol or sildenafil+tramadol impact negatively on testicular histology with poorreversal following withdrawal of treatment. |
