Measuring Tau-microtubule affinity through cosedimentation assays

Autor: Emily O, Alberico, Aranda R, Duan, Holly V, Goodson
Rok vydání: 2017
Předmět:
Zdroj: Methods in cell biology. 141
ISSN: 0091-679X
Popis: Microtubule cosedimentation assays have long been used to study the affinity of interactions between Tau protein and microtubules. While these assays are very useful for characterizing and comparing the effects of alterations to either Tau or the microtubule filaments, they can also be problematic. We provide a set of straightforward instructions for performing these assays and point out a number of challenges and pitfalls that can complicate their interpretation.
Databáze: OpenAIRE