| Autor: |
Bas G J, Surewaard, Krzysztof, Trzciński, Shamir R, Jacobino, Ivo S, Hansen, Mignon M, Vughs, Elisabeth A M, Sanders, Arie, van der Ende, Jos A G, van Strijp, Carla J C, de Haas |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Cellular microbiology. 15(10) |
| ISSN: |
1462-5822 |
| Popis: |
Neutrophil recruitment is essential in clearing pneumococcal infections. The first step in neutrophil extravasation involves the interaction between P-selectin on activated endothelium and P-Selectin Glycoprotein 1 (PSGL-1) on neutrophils. Here, we identify pneumococcal Zinc metalloproteinase C as a potent inhibitor of PSGL-1. ZmpC degrades the N-terminal domain of PSGL-1, thereby disrupting the initial rolling of neutrophils on activated human umbilical vein endothelial cells. Furthermore, mice infected with wild-type strain in the model of pneumococcal pneumonia showed lower lungs neutrophil infiltration compare to animals infected with ZmpC mutant. In addition, we confirmed the association of zmpC with serotype 8 and 11A and found it to be associated with serotype 33F as well. In conclusion, wereport PSGL-1 as a novel target for ZmpC and show that ZmpC inhibits neutrophil extravasation during pneumococcal pneumonia. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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