| Autor: |
D A, Kostiuchenko, V I, Kostiuchenko, G Ia, Kolomiĭtseva |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 47(8) |
| ISSN: |
0320-9725 |
| Popis: |
The structure of chromatin in the growing myxomycetes P. polycephalum can be interpreted on the basis of three types of substructure: (i) soluble in a low ionic strength buffer and 2 mM EDTA (I), (ii) soluble in 0.6 M NaCl (II) and (iii) non-soluble and nuclease-insensitive (III). The ratio of these structures in the nuclear pellet obtained after autolysis was found to be 71 : 0, 4 : 12 for the young and 7.7 : 62 : 20.6 for the old cultures, respectively. The ageing of the culture involves transition of substructure I to substructure II. The substructures differ in the nuclease sensitivity. The specific features of enzyme fragmentation of chromatin from P. polycephalum microplasmodium nuclei are discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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