[The development and use of an immunoenzyme test system for detecting and the species identification of the monkey pox virus]

Autor:	S S, Marennikova, O A, Zhukova, F G, Nagieva, E M, Shelukhina, G R, Matsevich
Rok vydání:	1991
Předmět:	Immunoenzyme Techniques Immunoglobulin Isotypes Virus Cultivation Immunoglobulin M Species Specificity Evaluation Studies as Topic Immunoglobulin G Animals Antibodies Monoclonal Humans Chick Embryo Monkeypox virus Antigens Viral
Zdroj:	Zhurnal mikrobiologii, epidemiologii i immunobiologii. (2)
ISSN:	0372-9311
Popis:	An enzyme immunoassay (EIA) system for the species-specific diagnosis of monkeypox, based on the use of monoclonal antibodies (McAb) to monkeypox virus, has been developed. Immunoglobulins, isolated from McAb-containing cultural and immune ascitic fluids, have been conjugated with horse-radish peroxidase and used as detector antibodies. For immunosorption, rabbit polyclonal antibodies to the vaccine virus have been used. The specificity and sensitivity of the EIA system thus obtained have been tested on animals and humans having monkeypox and confirmed by traditional diagnostic methods (the isolation of the virus on chick embryo chorioallantoic membranes and in cell culture).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::11a6e83604baafa1771d826cab681c3f https://pubmed.ncbi.nlm.nih.gov/1651036 Zobrazit plný text záznamu