Expression and cellular localization of Atrab28 during arabidopsis embryogenesis
| Autor: | C, Arenas-Mena, M, Raynal, A, Borrell, F, Varoquaux, M C, Cutanda, R A, Stacy, M, Pagès, M, Delseny, F A, Culiáñez-Macià |
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| Rok vydání: | 1999 |
| Předmět: |
Transcriptional Activation
DNA Complementary Sequence Homology Amino Acid Arabidopsis Proteins Molecular Sequence Data Arabidopsis Gene Expression Regulation Developmental Water Sequence Analysis DNA Genes Plant Immunohistochemistry Gene Expression Regulation Plant Growth Regulators Gene Expression Regulation Plant Multigene Family Tissue Distribution Amino Acid Sequence RNA Messenger Sequence Alignment In Situ Hybridization Abscisic Acid Plant Proteins Transcription Factors |
| Zdroj: | Plant molecular biology. 40(2) |
| ISSN: | 0167-4412 |
| Popis: | The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)+ stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies. |
| Databáze: | OpenAIRE |
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