| Popis: |
Macrophages are involved in the pathogenesis of mesangioproliferative glomerulonephritis. As macrophages are known to produce interleukin-10 (IL-10), we investigated the effect of recombinant murine IL-10 (rIL-10) on mesangial cell growth. In vitro studies were performed using the rat 1097 mesangial cell line. These cells exhibited a dose-dependent proliferative response to rIL-10 (23% to 70% increases at 80 ng/mL; p0.01), as assessed by both 3H-thymidine uptake and cell count. This effect was inhibited by preincubation of rIL-10 with a neutralizing anti-IL-10 antibody. When added to cultures of growth-arrested 1097 cells, IL-10 induced dose-dependent proliferation that paralleled the effects of platelet-derived growth factor. Incubation with a neutralizing anti-IL-10 Ab for 48 hours reduced 3H-thymidine uptake (median, 27% decreases; range, 2% to 56% decreases) versus a control Ab; p0.05). Rat mesangial cells were also shown to express IL-10 mRNA and protein, as determined by Northern blotting and immunostaining, thereby suggesting a role for IL-10 in autocrine mesangial cell growth. To examine the effects of IL-10 in vivo, inbred male Sprague-Dawley rats were given subcutaneous rIL-10 (0.5 mg/kg) for 3 (n = 6), 7 (n = 3), or 14 days (n = 4), or vehicle control, then killed. IL-10 administration induced a transient reduction in creatinine clearance of 35% at Day 3 (p0.01). Following IL-10 administration, an increase in glomerular cellularity was seen, which was maximal at Day 3 (82.7 +/- 5.9 nuclei/glomerular cross section versus control 64.6 +/- 4.6, 28% increases; p0.001) and maintained at Day 14 (23% increases; p0.01). Immuno-histochemical staining for proliferating cell nuclear antigen demonstrated an increased number of proliferating cells per glomerular cross section at day 3 (48% increases versus controls; p0.05). Staining for alpha-smooth-muscle actin showed significant labeling only in the glomeruli of IL-10-treated animals; double-labeling with an anti-proliferating cell nuclear antigen Ab demonstrated that some of these mesangial cells were proliferating. Collectively, these results suggest that IL-10 is a growth factor for rat mesangial cells both in vitro and in vivo. |
