| Autor: |
Xiao-bing, Li, Fu-guo, Shi, Xiao-jing, He, Ling-yan, Jian, Li, Ding |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Yao xue xue bao = Acta pharmaceutica Sinica. 51(3) |
| ISSN: |
0513-4870 |
| Popis: |
In this study, we developed a sensitive and rapid HPLC-MS/MS method for the determination of trans-ferulic acid(trans-FA) in plasma samples, and investigated the pharmacokinetics characteristics in healthy volunteers. The plasma samples were extracted with acetic ether, and then separated on a Hedera ODS-2 column with a mobile phase of methanol and 5 mmol·L(-1) ammonium acetate buffer solution containing 0.05% acetic acid(34∶66) at a flow rate of 0.4 m L·min(-1). Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited a good linearity over the concentration range of 0.1-5 ng·m L(-1)(r ≥ 0.999 2). The values on both the occasions(intra- and inter-day) were all within 9.2%, and the accuracy was 95.4%-111.4%. No matrix effect and carry-over effect were observed. Trans-FA was stable in human plasma under different storage conditions. The developed HPLC-MS/MS method is rapid, sensitive, accurate, and reproducible, and suitable for the pharmacokinetic study of trans-FA in healthy Chinese volunteers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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