[Effect of baicalin on signal transduction and activating transcription factor expression in ulcerative colitis patients]
| Autor: | Feng-yan, Yu, Shao-gang, Huang, Hai-yan, Zhang, Hong-gang, Chi, Ying, Zou, Ru-xi, Lu, Xue-bao, Zheng |
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| Rok vydání: | 2015 |
| Předmět: |
Flavonoids
Interleukin-6 Anti-Inflammatory Agents Non-Steroidal Blotting Western Activating Transcription Factors Interleukin-10 Irritable Bowel Syndrome Leukocytes Mononuclear Cytokines Humans Colitis Ulcerative Interleukin-4 Medicine Chinese Traditional Phosphorylation STAT6 Transcription Factor Signal Transduction |
| Zdroj: | Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine. 35(4) |
| ISSN: | 1003-5370 |
| Popis: | To explore the intervention of baicalin on signal transduction and activating transcription factor expression of ulcerative colitis (UC) patients.Recruited were UC patients at Outpatient Department of Digestive Disease, Inpatient Department of Digestive Disease, Center for Digestive Endoscopy of College City Branch, Guangdong Provincial Hospital of Traditional Chinese Medicine, and Southern Hospital affiliated to Southern Medical University from June 2010 to January 2011. They were assigned to the UC group (33 cases) and the diarrhea-predominant irritable bowel syndrome (IBS-D) group (30 cases). Another 30 healthy subjects were recruited as a healthy control group. Peripheral blood mononuclear cells (PBMCs) in vitro intervened by different concentrations baicalin were taken from UC patients. IL23R gene expressions in vitro intervened by different concentrations baicalin were detected using Q-PCR. Expressions of signal transducer and activator of transcription 4 (STAT4) , STAT6, phosphorylated-STAT4 (p-STAT4), and p-STAT6 were detected using Western blot. Serum levels of IFN-γ, IL-4, IL-6, and IL-10 were measured by ELISA. Effects of different concentrations baicalin on expressions of PBMCs, and levels of IFN-γ, IL-4, IL-10 of UC patients were also detected.Compared with the negative control group, 40 µmol baicalin obviously decreased IL23R gene expression of UC patients (P0. 01). Compared with the healthy control group and the IBS-D group, p-STAT4/STAT4 ratios increased, p-STAT6/STAT6 ratios decreased, levels of IFN-γ, IL-4, IL-10 all increased in the US group (all P0. 05). Compared with the negative control, 5 and 10 µmol baicalin groups, 20 and 40 moL baicalin obviously decreased p-STAT4/STAT4 ratios (all P0. 05); 20 and 40 µmoL baicalin obviously increased p-STAT6/STAT6 ratios (all P0. 05); 20 and 40 µmoL baicalin obviously lowered levels of IFN-γ and IL-4, and elevated IL-10 levels (all P0. 05).40 µmoL baicalin could in vitro inhibit p-STAT4/STAT4 ratios, adjust p-STAT6/STAT6 ratios and related cytokines, thereby balancing the immunity and relieving inflammatory reactions of UC. |
| Databáze: | OpenAIRE |
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