Resveratrol inhibits plasma membrane Ca
| Autor: | Joshua Allen, Peterson, Richard Vernon, Oblad, Jeffrey Chad, Mecham, Jason Donald, Kenealey |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
TG
thapsigargin DMEM Dulbecco's modified Eagle medium PMCA plasma membrane Ca2+-ATPase HBSS Ca2+- and Mg2+-free Hank's Balanced Salt Solution ROI region of interest PBS phosphate-buffered saline Calcium signaling DMSO dimethyl sulfoxide [Ca2+]i cytosolic calcium concentration BAPTA BAPTA-Acetoxymethyl ester ER endoplasmic reticulum Fura-2 Fura-2-Acetoxymethyl ester Plasma membrane Ca2+-ATPase FBS fetal bovine serum RES resveratrol Resveratrol SERCA sarcoendoplasmic reticular Ca2+-ATPase Fura-2 EGCG epigallocatechin gallate Research Article |
| Zdroj: | Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| Popis: | Plasma membrane Ca2+-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca2+]i). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca2+]i via PMCA inhibition in primary dermal fibroblasts and MDA-MB-231 breast cancer cells. Our results also illustrate that RES and the fluorescent intracellular calcium indicator Fura-2, are compatible for simultaneous use, in contrast to previous studies, which indicated that RES modulates the Fura-2 fluorescence independent of calcium concentration. Because RES has been identified as a PMCA inhibitor, further studies may be conducted to develop more specific PMCA inhibitors from RES derivatives for potential therapeutic use. Highlights • Resveratrol induces a rise in [Ca2+]i via plasma membrane Ca2+-ATPase inhibition. • FURA-2 is compatible with resveratrol in measuring [Ca2+]i. • PMCA inhibition is novel to resveratrol among naturally occurring polyphenols. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|