Transforming growth factor-β2 increases extracellular matrix proteins in optic nerve head cells via activation of the Smad signaling pathway
| Autor: | Zode, Gulab S., Sethi, Anirudh, Brun-Zinkernagel, Anne-Marie, Chang, I-Fen, Clark, Abbot F., Wordinger, Robert J. |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Aged
80 and over Extracellular Matrix Proteins genetic structures Reverse Transcriptase Polymerase Chain Reaction Blotting Western Optic Disk Glaucoma Smad Proteins Immunohistochemistry eye diseases Extracellular Matrix Transforming Growth Factor beta2 Astrocytes Case-Control Studies Humans sense organs Gene Silencing RNA Messenger Phosphorylation RNA Small Interfering Extracellular Signal-Regulated MAP Kinases Cells Cultured Research Article Aged Signal Transduction |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose Transforming growth factor-β2 (TGF-β2) is associated with glaucomatous neuropathy, primarily via the increased synthesis and secretion of extracellular matrix (ECM) proteins and remodeling of the optic nerve head (ONH). Here, we investigated the signaling pathways used by TGF-β2 to stimulate ECM expression by ONH astrocytes and lamina cribrosa (LC) cells. Methods TGF-β2 localization and secretion was examined in human donor tissues and ONH astrocytes and LC cells. To examine TGF-β2 signaling, ONH astrocytes and LC cells were treated with recombinant TGF-β2, and phosphorylation of Smad and non-Smad signaling proteins were examined by western blot analyses and immunostaining. Results TGF-β2 is significantly increased in the LC region of the ONH in glaucomatous eyes compared to age-matched normal eyes (n=4, p |
| Databáze: | OpenAIRE |
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