| Autor: |
H P, Dyadyusha, O L, Sanina |
| Rok vydání: |
1975 |
| Předmět: |
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| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal. 47(2) |
| ISSN: |
0041-610X |
| Popis: |
It is shown that simultaneous treatment with sarcolemma with triton X-100 (0.1-1.0%) and KCl (0.6-1.2 M) solubilizes about 60% of acetyl cholinesterase, however its specific activity in the intact sarcolemma and in the obtained fractions is the same. A considerable part of lipids (about 90% of phospholipids and 50% of cholesterol) may be removed by treating sarcolemma with 0.1% triton X-100; it may be fixed in the membrane by hydrophoric bonds. Other phospholipids and cholesterol are bound with sarcolemma proteins more strongly and, probably, form the protein-lipid complexes. In the composition of phospholipids strongly bound with sarcolemma proteins a relative content of sphingomyelin increases and that of lysolecithin decreases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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