| Autor: |
Xiong-Bing, Lu, Hui-Li, Xie, Hong-Wei, Huang, Zi-Min, Shi, Jian-Hua, Xiong, Zheng-Yue, Pan |
| Rok vydání: |
2006 |
| Předmět: |
|
| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 22(6) |
| ISSN: |
1007-8738 |
| Popis: |
To induce dendritic cells (DCs) from human peripheral blood monocytes in vitro and to investigate the kinetics lymphotactin (Lptn) mRNA expression.Monocytes were isolated from normal human peripheral blood cells by density gradient centrifugation and the plastic-adherent cells were cultured with the cytokines (rhGM-CSF, rhIL-4, rhTNF-alpha). The immature and mature DCs' surface antigens CD1a and CD83 were analyzed by flow cytometry (FCM). The morphology of mature DCs induced by rhTNF-alpha was observed under electron microscope. Lptn cDNA was amplified by RT-PCR, cloned to vector pGM-T Easy and completely sequenced. The RT-PCR products of cells were separated on gel and analyzed by semi-quantification.The cells cultured for 7 d exhibited special dendritic morphology of mature DCs and highly expressed CD83. The sequence of cDNA was confirmed and was identical to that of NO. U23772 published in GenBank. The Lptn mRNA expression of DCs was stronger in DCs cultured for 7 d than in those for 5 d, whereas it was not detected in those for 3 d.The monocyte-derived dendritic cells can express Lptn mRNA in a maturation-dependent manner. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
