| Autor: |
Liliya, Vugmeyster, Aaron, Griffin, Dmitry, Ostrovsky, Shibani, Bhattacharya, Parker J, Nichols, C James, McKnight, Beat, Vögeli |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Journal of biomolecular NMR. 72(1-2) |
| ISSN: |
1573-5001 |
| Popis: |
We investigated correlated μs-ms time scale motions of neighboring (13)C’─(15)N and (13)C(α)─(13)C(β) nuclei in both protonated and perdeuterated samples of GB3. The techniques employed, NMR relaxation due to cross-correlated chemical shift modulations, specifically target concerted changes in the isotropic chemical shifts of the two nuclei associated with spatial fluctuations. Field-dependence of the relaxation rates permits identification of the parameters defining the chemical exchange rate constant under the assumption of a two-site exchange. The time scale of motions falls into the intermediate to fast regime (with respect to the chemical shift time scale, 100 to 400 s(−1) range) for the (13)C’─(15)N pairs and into the slow to intermediate regime for the (13)C─α(13)C(β)pairs ( about 150 s(−1)) . Comparison of the results obtained for protonated and deuterated GB3 suggests that deuteration has a tendency to reduce these slow scale correlated motions, especially for the (13)C(α)─(13)C(β) pairs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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