Rhizobium radiobacter conjugation and callus-independent shoot regeneration used to introduce the cercosporin export gene cfp from Cercospora into sugar beet (Beta vulgaris L.)

Autor:	L David, Kuykendall, Tammy M, Stockett, Joseph W, Saunders
Rok vydání:	2003
Předmět:	Membrane Transport Proteins Cell Differentiation Plants Genetically Modified Recombinant Proteins Fungal Proteins Transformation Genetic Ascomycota Conjugation Genetic Culture Techniques Regeneration Beta vulgaris Carrier Proteins Perylene Plant Shoots Plant Diseases Rhizobium
Zdroj:	Biotechnology letters. 25(9)
ISSN:	0141-5492
Popis:	Leaf spot disease caused by Cercospora is responsible for crop and profitability losses in sugar beet crops in the US and worldwide. The cfp gene that encodes a protein that exports phytotoxic cercosporins from Cercospora was conjugally transferred to sugar beet using Rhizobium radiobacter (Agrobacterium tumefaciens), to improve Cercospora-induced leafspot resistance. Conditions for shoot regeneration were optimized to increase regeneration/transformation efficiencies. Low-light and room-temperature conditions were favorable to sugar beet regeneration without callus when cytokinin had been added to the tissue culture medium. Using this procedure adventitious shoots from leaf pieces were obtained in a simple, one-step regeneration procedure. T7, a cfp-transgenic clone verified by PCR with gene-specific primers, is being propagated for leaf spot disease resistance evaluation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0f255767ffc1004a136cc4652bd7fc55 https://pubmed.ncbi.nlm.nih.gov/12882176 Zobrazit plný text záznamu Full text from SpringerLink