| Autor: |
Pil Soo, Sung, Dong Jun, Park, Pu Reun, Roh, Kyoung Do, Mun, Sung Woo, Cho, Gil Won, Lee, Eun Sun, Jung, Sung Hak, Lee, Jeong Won, Jang, Si Hyun, Bae, Jong Young, Choi, Jonghwan, Choi, Jaegyoon, Ahn, Seung Kew, Yoon |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal for immunotherapy of cancer. 10(5) |
| ISSN: |
2051-1426 |
| Popis: |
IgA neutralizes pathogens to prevent infection at mucosal sites. However, emerging evidence shows that IgA contributes to aggravating inflammation or dismantling antitumor immunity in human diseased liver. The aim of this study was to elucidate the roles of inflammation-induced intrahepatic inflammatory IgAPatient cohorts including steatohepatitis cohort (n=61) and HCC cohort (n=271) were established. Patients' surgical and biopsy specimens were analyzed using immunohistochemistry. Multicolor flow cytometry was performed with a subset of patient samples. Single-cell RNA-Seq analysis was performed using Gene Expression Omnibus (GEO) datasets. Additionally, we performed in vitro differentiation of macrophages, stimulation with coated IgA, and RNA sequencing. Hepa1-6 cells and C57BL/6N mice were used to obtain HCC syngeneic mouse models.Serum IgA levels were associated (p0.001) with fibrosis progression and HCC development in patients with chronic liver diseases. Additionally, immunohistochemical staining of inflamed livers or HCC revealed IgA positivity in monocytes, with a correlation between IgAOverall, the findings of this study showed that serum IgA levels was correlated with intrahepatic and intratumoral infiltration of inflammatory IgA |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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