Relationship of multidrug resistance to rhodamine-123 selectivity between carcinoma and normal epithelial cells: taxol and vinblastine modulate drug efflux
| Autor: | D, Brouty-Boyé, D, Kolonias, C J, Wu, N, Savaraj, T J, Lampidis |
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| Rok vydání: | 1995 |
| Předmět: |
Base Sequence
Paclitaxel Cell Survival Rhodamines Molecular Sequence Data Gene Expression Breast Neoplasms Epithelial Cells Vinblastine Polymerase Chain Reaction Drug Resistance Multiple Epithelium Cell Line Tumor Cells Cultured Humans Female Rhodamine 123 ATP Binding Cassette Transporter Subfamily B Member 1 Breast DNA Primers |
| Zdroj: | Cancer research. 55(8) |
| ISSN: | 0008-5472 |
| Popis: | Preferential retention and cytotoxicity of Rhodamine-123 (Rho-123) was originally reported in a number of carcinoma cell types isolated from a variety of tissues as compared to normal epithelial cells from a limited number of other tissues. In the present study, we have examined Rho-123 selectivity in normal and tumor cell lines isolated from the same tissue source, i.e., human breast. We found that: (a) in matched pairs of normal and carcinoma breast cells, Rho-123 displays no preferential retention in either cell type; (b) there is no preferential toxicity in carcinoma as compared to normal breast cells; in fact, one of the carcinoma cell lines (MDA-MB231) shows moderate resistance to this dye; (c) all of the human breast cell lines do not express P-glycoprotein-mediated multidrug resistance; (d) the normal monkey kidney epithelial cell line CV-1, which was originally used as a model to demonstrate the relative resistance of normal epithelial cells to this drug, is found to express high levels of the mdr-1 gene, is resistant to other multidrug-resistant drugs (taxol and vinblastine), and its resistance to Rho-123 as well as decreased Rho-123 retention can be reversed by verapamil; and (e) taxol and vinblastine are found to block increased Rho-123 efflux in CV-1 cells. Thus, overall the data suggest that preferential retention and cytotoxicity of Rho-123 in carcinoma versus normal epithelial cells is related to the differential expression of the mdr-1 gene. |
| Databáze: | OpenAIRE |
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