CD11b
| Autor: | Gentaro, Izumi, Hideki, Nakano, Keiko, Nakano, Gregory S, Whitehead, Sara A, Grimm, Michael B, Fessler, Peer W, Karmaus, Donald N, Cook |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
T-helper 2 cells Article Mice Th2 Cells Animals T-helper 17 cells Lung Cells Cultured Lymphocyte activation Mice Knockout CD11b Antigen Base Sequence urogenital system Cell Differentiation Dendritic Cells Adoptive Transfer Asthma Coculture Techniques Mice Inbred C57BL Disease Models Animal Conventional dendritic cells embryonic structures Th17 Cells Mucosal immunology biological phenomena cell phenomena and immunity Single-Cell Analysis |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Dendritic cells (DC) in the lung that induce Th17 differentiation remain incompletely understood, in part because conventional CD11b+ DCs (cDC2) are heterogeneous. Here, we report a population of cDCs that rapidly accumulates in lungs of mice following house dust extract inhalation. These cells are Ly-6C+, are developmentally and phenotypically similar to cDC2, and strongly promote Th17 differentiation ex vivo. Single cell RNA-sequencing (scRNA-Seq) of lung cDC2 indicates 5 distinct clusters. Pseudotime analysis of scRNA-Seq data and adoptive transfer experiments with purified cDC2 subpopulations suggest stepwise developmental progression of immature Ly-6C+Ly-6A/E+ cDC2 to mature Ly-6C–CD301b+ lung resident cDC2 lacking Ccr7 expression, which then further mature into CD200+ migratory cDC2 expressing Ccr7. Partially mature Ly-6C+Ly-6A/E–CD301b– cDC2, which express Il1b, promote Th17 differentiation. By contrast, CD200+ mature cDC2 strongly induce Th2, but not Th17, differentiation. Thus, Th17 and Th2 differentiation are promoted by lung cDC2 at distinct stages of maturation. Dendritic cells in the lung may be specialised to mediate specific types of immune function. Here the authors show that subpopulations of mouse CD11b+ lung DC at different stages of maturation and phenotype can promote Th17 or Th2 CD4+ T cell differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|