[Optimization of conditions for storage and cultivation of the fungus Claviceps sp.--a producer of the ergot alkaloid agroclavine]

Autor: L V, Boĭchenko, N F, Zelenkova, M U, Arinbasarov, T A, Reshetilova
Rok vydání: 2003
Předmět:
Zdroj: Prikladnaia biokhimiia i mikrobiologiia. 39(3)
ISSN: 0555-1099
Popis: Conditions of agroclavine biosynthesis by the mutant Claviceps sp. strain s 106 were studied. The content of agroclavine was maximum (1.5-2 g/l) on days 15-16 of cultivation in the complex medium T25, containing sucrose, citric acid, and yeast extract. Agroclavine was the major component of the alkaloid fraction (90-95%). Storage of the culture at -70 degrees C in T25 supplemented by 7% glycerol provided a stable level of alkaloid formation.
Databáze: OpenAIRE