Ribosomal subunit antiassociation activity in rabbit reticulocyte lysates. Evidence for a low molecular weight ribosomal subunit antiassociation protein factor (Mr = 25,000)

Autor:	P, Raychaudhuri, E A, Stringer, D M, Valenzuela, U, Maitra
Rok vydání:	1984
Předmět:	Molecular Weight Ribosomal Proteins RNA Transfer Met Reticulocytes Peptide Initiation Factors Eukaryotic Initiation Factor-3 Chromatography Gel Animals Electrophoresis Polyacrylamide Gel Magnesium Rabbits RNA Transfer Amino Acyl Chromatography DEAE-Cellulose
Zdroj:	The Journal of biological chemistry. 259(19)
ISSN:	0021-9258
Popis:	A ribosomal subunit antiassociation activity has been purified from both the postribosomal supernatant and ribosomal salt-wash protein fractions of rabbit reticulocyte lysates. A majority (greater than 90%) of the activity is associated with a low molecular weight protein of Mr of approximately 25,000. A small but significant level of antiassociation activity (less than 10%) was found to be associated with higher molecular weight protein fractions. The purified 25,000-dalton antiassociation factor interacts with 60 S ribosomal subunits to prevent them from reassociating with 40 S ribosomal subunits. The factor does not seem to interact directly with 40 S subunits nor does it dissociate 80 S monosomes. The properties of this factor are thus similar to the eukaryotic initiation factor 6 isolated from both wheat germ and calf liver extracts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0e6c3957314d987174ff21384c14e564 https://pubmed.ncbi.nlm.nih.gov/6566675 Zobrazit plný text záznamu