High lymphatic vessel density correlates with overexpression of VEGF-C in gastric cancer
| Autor: | Takashi, Hachisuka, Michihiro, Narikiyo, Yukishige, Yamada, Hirofumi, Ishikawa, Masato, Ueno, Hideki, Uchida, Ryo, Yoriki, Yuichirou, Ohigashi, Katsuhiko, Miki, Hidetoshi, Tamaki, Takashi, Mizuno, Yoshiyuki, Nakajima |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Neovascularization Pathologic Reverse Transcriptase Polymerase Chain Reaction Microcirculation Vascular Endothelial Growth Factor C Vesicular Transport Proteins Antigens CD34 Middle Aged Prognosis Immunohistochemistry Stomach Neoplasms Cell Line Tumor Lymphatic Metastasis Disease Progression Humans Female RNA Messenger Lymphangiogenesis Aged Glycoproteins Lymphatic Vessels |
| Zdroj: | Oncology reports. 13(4) |
| ISSN: | 1021-335X |
| Popis: | Lymph node metastasis is one of the most important prognostic factors in malignant tumors. In this study, we investigated vascular endothelial growth factor (VEGF)-C expression in human gastric cancer using immunohistochemical techniques and determined the number of microvessels in peritumoral tissue. VEGF-C expression was positive in 22 of 79 cases (27.8%), and correlated with the presence of lymphatic invasion and lymph node metastasis. We confirmed by reverse transcription-polymerase chain reaction (RT-PCR) that VEGF-C mRNA expression is observed more commonly in cancer tissues than normal tissues. For 59 gastric tumors, we examined lymphatic vessel density (LVD) using the specific lymphatic vessel endothelial hyaluronan receptor (LYVE) -1 antibody. VEGF-C expression was observed in 10 of 25 cases (40%) that exhibited a high LVD. Furthermore, high LVD exhibited a significant correlation with VEGF-C expression. Our findings suggest that VEGF-C plays a pivotal role for lymphangiogenesis and tumor growth in gastric cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|