Expression of p33ING1 mRNA and chemosensitivity in brain tumor cells
| Autor: | G, Tallen, K, Riabowol, J E, Wolff |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Paclitaxel
Cell Cycle Proteins Nerve Tissue Proteins Microtubules Predictive Value of Tests Humans Genes Tumor Suppressor RNA Messenger RNA Neoplasm Enzyme Inhibitors Etoposide Brain Neoplasms Tumor Suppressor Proteins Intracellular Signaling Peptides and Proteins Nuclear Proteins Proteins Glioma Fibroblasts Neoplasm Proteins DNA-Binding Proteins Doxorubicin Drug Resistance Neoplasm Vincristine Protein Biosynthesis Cisplatin Topoisomerase I Inhibitors Biomarkers Inhibitor of Growth Protein 1 DNA Damage Medulloblastoma |
| Zdroj: | Anticancer research. 23(2B) |
| ISSN: | 0250-7005 |
| Popis: | Mutations and down-regulation of tumor suppressor genes can contribute to both tumorigenesis and chemotherapy resistance. The tumor suppressor p33ING1 has growth-inhibitory and pro-apoptotic effects recruiting p53 and it plays a role in DNA repair through interaction with PCNA. We questioned whether p33ING1 mRNA expression correlates with the chemosensitivity of brain tumor cells.Various malignant brain tumor cell lines were examined for their sensitivity to cisplatin, doxorubicin, etoposide and the antimitotic agents vincristine and paclitaxel by MTT-cytotoxicity assays. p33ING1 mRNA expression was determined by RT-PCR.We found that, unlike other tumor types, ING1 levels were higher in glioma cell lines than in normal control cells. Medulloblastoma cells revealed the lowest ING1 expression of the lines tested. Comparing all cell lines, p33ING1 gene expression significantly (p = 0.028) correlated with resistance to vincristine (r2 = 0.87).Our results suggest that p33ING1 mRNA levels may be used to predict the chemosensitivity of brain tumor cells to vincristine. |
| Databáze: | OpenAIRE |
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