Reduced memory B-cell populations in boys with B-cell dysfunction after bone marrow transplantation for X-linked severe combined immunodeficiency
Autor: | S S, Ting, S G, Tangye, J, Wood, R A, Ffrench, J B, Ziegler |
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Rok vydání: | 2001 |
Předmět: |
Male
B-Lymphocytes X Chromosome Antigens CD19 Infant Antigens CD34 Immunoglobulin D CD5 Antigens Flow Cytometry Statistics Nonparametric Immunophenotyping Tumor Necrosis Factor Receptor Superfamily Member 7 Immunoglobulin M Agammaglobulinemia Case-Control Studies Immunoglobulin G Humans Neprilysin Severe Combined Immunodeficiency Immunologic Memory Bone Marrow Transplantation Follow-Up Studies |
Zdroj: | British journal of haematology. 112(4) |
ISSN: | 0007-1048 |
Popis: | X-linked severe combined immunodeficiency (XSCID) is a lethal disease resulting in death in infancy. In many instances, haploidentical bone marrow transplantation (BMT) offers reconstitution of T-cell immunity alone, with residual hypogammaglobulinaemia. The exact nature of B-cell dysfunction in these patients is unclear, although differentiation arrest of the B cells is a potential explanation. To ascertain the differentiation status of peripheral blood B lymphocytes from XSCID patients after BMT, the surface expression of CD19, CD10, CD34, CD5, serum immunoglogulin (sIg)M, sIgD, sIgG and CD27 on these B cells was investigated using three-colour flow cytometry. CD27 is a marker of memory B cells. Populations of CD19+IgM-D- B cells, CD19+IgM-only, CD19+IgG+CD27+ and CD19+IgM+ CD27+ B cells were found to be diminished in the XSCID patients after BMT with persistent hypogammaglobulinaemia, compared with both post-BMT patients with B-cell function and age-matched normal controls. This indicated the lack of CD19+IgM-D- B cells, which represent Ig isotype-switched B cells, as well as CD19+IgM-only and CD19+IgG+CD27+ or CD19+IgM+CD27+ memory B-cell populations. Interaction between CD27 and its ligand CD70 has been shown to induce IgG and IgM production by CD27+ B cells. Therefore, the lack of CD27/70 interaction is a probable explanation for the hypogammaglobulinaemia in these patients after BMT. |
Databáze: | OpenAIRE |
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