| Autor: |
Helen F, Downie, Joel P, Standerwick, Letitia, Burgess, Louise S, Natrajan, Jonathan R, Lloyd |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Research in microbiology. 169(10) |
| ISSN: |
1769-7123 |
| Popis: |
Dissimilatory iron-reducing bacteria (DIRB) play an important role in controlling the redox chemistry of Fe and other transition metals and radionuclides in the environment. During bacterial iron reduction, electrons are transferred from the outer membrane to poorly soluble Fe(III) minerals, although the precise physiological mechanisms and local impact on minerals of these redox processes remain unclear. The aim of this work was to use a range of microscopic techniques to examine the local environment of Geobacter sulfurreducens grown on thin films of Fe(III)-bearing minerals, to provide insight into spatial patterns of Fe(III) reduction and electron transfer. Confocal fluorescence microscopy showed that sparse biofilms formed on the mineral coatings, while the selective Fe(II) probe RhoNox-1 revealed Fe(II) patches on the minerals sometimes co-located with cells. Atomic force microscopy highlighted thin filamentous structures extending radially from the cell surface. Further analysis using fluorescent redox dyes showed redox-active, linear nanowires that formed cell to cell connections, although they were not implicated in playing a dominant role in direct electron transfer to the Fe(III) minerals. Overall this paper provides new methods and insights on studying Fe(III) reduction and other redox transformations in situ. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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