| Autor: |
Dorota, Żakowska, Grzegorz, Graniak, Paweł, Rutyna, Katarzyna, Naylor, Patrycja, Głowacka, Marcin, Niemcewicz |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Annals of agricultural and environmental medicine : AAEM. 26(3) |
| ISSN: |
1898-2263 |
| Popis: |
Existing research for using the protective antigen (PA) ofiBacillus anthracis/ias a vaccine component shows that protection against anthrax may be obtained using fragments of this protein. The aim of the research is to check whether the selected protein fragment of the protective antigen (domain 4) encoded by an appropriate nucleotide sequence of gene pag ofiB. anthracis/i, was expressed in the bacterial system ofiE. coli/i. In order to examine the selected sequence of the pag gene, a PCR reaction and a highly effective TOPO cloning strategy were used, followed by purification of the recombinant proteins and their detection by a western-blot method. In the planning of the PA4 antigen expression a higher level of effectiveness in production of small protein - domain 4 - was anticipated. As a result, the 139 amino acids protein fragment ofiB. anthracis/iPA (domain 4) was isolated. The research may have found the basis for in vivo research aimed at finding potential anthrax vaccine components. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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