| Autor: |
Nirmala, Padmanabhan, Tabrez J, Siddiqui |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1538 |
| ISSN: |
1940-6029 |
| Popis: |
This protocol describes an in situ protein-protein interaction assay between tagged recombinant proteins and cell-surface expressed synaptic proteins. The assay is arguably more sensitive than other traditional protein binding assays such as co-immunoprecipitation and pull-downs and provides a visual readout for binding. This assay has been widely used to determine the dissociation constant of binding of trans-synaptic adhesion proteins. The step-wise description in the protocol should facilitate the adoption of this method in other laboratories. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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