[Enhancement of HSP-MUC1 antitumor activity by type C CpG-ODN BW005]
| Autor: | Xue-Ju, Wang, Zhi-Bo, Wang, Hong-Fei, Wei, Xiu-Li, Wu, Li, Wang, Yong-Li, Yu, Li-Ying, Wang |
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| Rok vydání: | 2007 |
| Předmět: |
Mucin-1
Antineoplastic Agents Enzyme-Linked Immunosorbent Assay Flow Cytometry Transfection Cell Line Mice Inbred C57BL Survival Rate Disease Models Animal Mice Random Allocation Oligodeoxyribonucleotides Immunoglobulin G Neoplasms Chlorocebus aethiops Animals Humans Female Vero Cells Heat-Shock Proteins Cell Line Transformed Cell Proliferation |
| Zdroj: | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 23(4) |
| ISSN: | 1007-8738 |
| Popis: | To investigate the enhancement of anti-tumor effect of HSP-MUC1 by self-designed type C CpG-ODN BW005.The immunostimulatory effect of CpG-ODN BW005 was detected by IFN protection assay and (3)H proliferation assay in vitro. Sixty C57BL/6 mice were separated into 5 groups randomly, including Sodium Chloride control, HSP-MUC1 control, HSP-MUC1/1585, HSP-MUC1/1826 and HSP-MUC1/BW005. Mice were injected s.c. with agents on day 0, 14 and 28 and were implanted MUC1-EL4 tumor cells s.c. on day 33. Tumor growth and murine death were recorded. Blood was collected in 57 day from tail vein. Subtype of anti-HSP and anti-MUC1 IgG in serum was detected by indirect ELISA.CpG-ODN BW005 could stimulate the proliferation of hPBMC and mice spleoncyte and IFNalpha production. HSP-MUC1/BW005 postponed tumor development, with the average tumor-developed day of 44.8, and prolonged the survival of mice with the average survival day of 49.5. Moreover, final tumor-developed rate of this group was 33.33%, which was the lowest; final survival rate of this group was 66.67%, which was the highest. Levels of anti-HSP and anti-MUC1 IgG2a in HSP-MUC1/CpG-ODNs group were enhanced.CpG-ODN BW005, a kind of type C CpG-ODN, could enhance the anti-tumor effect of HSP-MUC1. |
| Databáze: | OpenAIRE |
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