| Autor: |
I N, Aleksandrova, I V, Koshkarova, A A, Lyshchev, K O, Sitskaia, L S, Zubarovskaia, S L, Akimova, L N, Shabanova, G F, Pluzhnikova, V I, Rudenko, K P, Khanson, B V, Afanas'ev |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Voprosy onkologii. 42(5) |
| ISSN: |
0507-3758 |
| Popis: |
Cytomegalovirus (CMV) infection often causes death after organ transplantation. Therefore, adequate prevention, diagnosis and treatment of this complication are of critical importance. The experience of establishing routine PCR-detection of CMV is presented. A widely accepted PCR protocol was used for clinical purposes. The strategy included 3 steps: 1) PCR amplification of CMV-sequences from leukocytes of patients; 2) visualization of PCR-product in polyacrylamide gel; 3) verification of the identity of PCR product by its hybridization to a membrane-bound specific probe. This approach allowed to detect CMV infection in 10 out of 28 bone marrow and in 4 out of 32 kidney recipients. The administration of specific antiviral treatment led to the elimination of CMV sequences from the blood. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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