| Autor: |
Xi-Zhi, Ma, Li-Jun, Cai, Xiao-Hui, Wu, Shou-Yuan, Zhao, Chang-Ben, Li, Ke-Jing, Deng |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 35(7) |
| ISSN: |
0582-9879 |
| Popis: |
Many human genes determined by genomic sequencing have only few information about their functions. To fill this knowledge gap, the powerful Drosophila genetics was set as a model to elucidate human gene functions effectively. By using germline transformation together with GAL4-UAS system, we studied the possibility of expressing and functionally characterization of human genes in Drosophila. Fifty-four transgenic fly lines corresponding to 10 human genes have been established. When expressed individually by crossing to an array of 6 different GAL4 driver lines, one of these genes, the translation elongation factor 1 alpha 1 (EF1 alpha-1), resulted in abnormal notum and rough eye phenotypes. This study implies the feasibility of systematically screening human gene functions by overexpression in Drosophila. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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