| Autor: |
M, Negrini, A, Castagnoli, S, Sabbioni, E, Recanatini, G, Giovannini, L, Possati, E J, Stanbridge, I, Nenci, G, Barbanti-Brodano |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Oncogene. 7(10) |
| ISSN: |
0950-9232 |
| Popis: |
Breast cancer development is associated with several genetic abnormalities. Loss of heterozygosity in the short arm of chromosome 11 has been observed in 30% of tumors. We found homozygosity at five chromosome 11 polymorphic loci in genomic DNA of the MCF-7 breast carcinoma cell line, suggesting a possible loss of one chromosome 11. We have studied the transformed and tumorigenic phenotypes of MCF-7 cells following introduction of a normal human chromosome 11 via microcell fusion. MCF-7/H11 cell hybrids, containing chromosome 11, showed in vitro characteristics similar to the parental cell line. However, tumorigenicity in athymic mice was completely suppressed. Since tumor formation by MCF-7 cells is estrogen dependent, we have analysed the expression of the estrogen receptor and of the estrogen-activated gene pS2. No difference was detected between the parental MCF-7 cells and the derived chromosome 11 cell hybrids, indicating that the mechanism of MCF-7 tumor suppression by chromosome 11-associated functions does not directly involve the estrogen/estrogen receptor molecular pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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