| Autor: |
Zi-fen, Guo, Lin-ling, Chen, Jia, Zhang, Cui-ying, Peng, Xiang-dong, Yang, Xu, Zhang, Shu-ya, He, Duan-fang, Liao, Kai, Li |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics. 20(4) |
| ISSN: |
1003-9406 |
| Popis: |
To determine whether 3'phosphorothioate-modified-2 terminal mismatched primers can turn off DNA polymerization mediated by Exo(+) polymerase.Two-directional primer extension was performed using polymerase with and without 3' exonuclease activity. The effects of unmodified primers and 3' phosphorothioate-modified primers on primer extension were evaluated.Exo(-) polymerase yielded products from matched and mismatched primers regardless of their modification. However, 3' phosphorothioate-modified primers with a single base mismatch at -2 position worked similarly to the terminal (-1) mismatched primers in triggering the novelly reported "off-switch" of Exo(+) polymerase.These data suggested that the "off-switch" can be of enormous application in the diagnosis of single gene diseases and in the association studies by single nucleotide polymorphism screening. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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