| Autor: |
Hidekazu, Tanaka, Retsu, Mitsui, Mitsuhiro, Oishi, Stefan, Passlick, Ronald, Jabs, Christian, Steinhäuser, Kenji F, Tanaka, Hikaru, Hashitani |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
British journal of pharmacologyREFERENCES. 178(5) |
| ISSN: |
1476-5381 |
| Popis: |
While the bladder vasculature is considered as a target of PDE5 inhibitors to improve bladder storage dysfunctions, its characteristics are largely unknown. Thus, the functional and morphological properties of arteries/arterioles of the bladder focusing on the NO-mediated signal transmission were explored.Diameter changes in rat bladder arteries/arterioles were measured using a video-tracking system. Intercellular CaIn rat suburothelial arterioles and vesical arteries, tadalafil (100 nM) attenuated nerve-evoked sympathetic vasoconstrictions. In both vascular segments, tadalafil-induced inhibition of sympathetic vasoconstriction was prevented by N ω-propyl-l-arginine hydrochloride (l-NPA, 1 μM), an nNOS inhibitor or N ω-nitro-l-arginine (l-NA, 100 μM). Both vascular segments were densely innervated with nNOS-positive nitrergic nerves in close apposition to tyrosine hydroxylase-immunoreactive sympathetic nerves. In pericyte-covered pre-capillary arterioles of the mouse bladder where sympathetic nerves were absent, nerve stimulation evoked transient reductions in pericyte CaBoth nitrergic nerve and nerve-evoked endothelial NO release appear to be involved in vasodilatory signal transmission in bladder vasculature. The NO-mediated signal transmission is a potential target for PDE5 inhibitor therapy in bladder dysfunctions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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