Free circulating DNA as a biomarker of prostate cancer: comparison of quantitation methods
| Autor: | Kavitha, Ramachandran, Carl G, Speer, Stephanie, Fiddy, Isildinha M, Reis, Rakesh, Singal |
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| Rok vydání: | 2013 |
| Předmět: |
Aged
80 and over Male Prostatic Neoplasms DNA Middle Aged Prostate-Specific Antigen Real-Time Polymerase Chain Reaction ROC Curve Multivariate Analysis Biomarkers Tumor Humans Kallikreins Spectrophotometry Ultraviolet Organic Chemicals Blood Chemical Analysis Early Detection of Cancer Aged Fluorescent Dyes |
| Zdroj: | Anticancer research. 33(10) |
| ISSN: | 1791-7530 |
| Popis: | To identify a simpler method of free circulating DNA (fcDNA) quantitation that may improve the specificity of the prostate cancer prostate-specific antigen (PSA) screening test.The patient group consisted of 241 men with elevated PSA/abnormal digital rectal exam (DRE), undergoing prostate biopsy. Serum fcDNA levels were measured by UV absorbance and PicoGreen. Results were compared to previously published quantitative polymerase chain reaction (qPCR) data.We found that levels of fcDNA measured by PicoGreen correlated well with those measured by qPCR (r=0.8552). In the patient group with PSA4 to 10 ng/ml, those with fcDNA (PicoGreen)53.1 ng/ml were at increased risk for prostate cancer compared to those with fcDNA ≤ 53.1 ng/ml. Moreover, we found that measuring fcDNA levels by PicoGreen does not compromise the negative predictive value, accuracy or specificity of the qPCR fcDNA test.If validated in larger studies, PicoGreen quantitation of fcDNA could serve as a simple method to aid in prostate cancer diagnosis. |
| Databáze: | OpenAIRE |
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