[Isolation and features of the functioning of mitochondria of the small intestine mucosa in the rat]

Autor: I V, Shostakovskaia, A M, Babskiĭ
Rok vydání: 1984
Předmět:
Zdroj: Ukrainskii biokhimicheskii zhurnal (1. 56(2)
ISSN: 0201-8470
Popis: A modification is suggested for the method of intact mitochondria isolation from rat thin intestine mucosa. Mitochondria are obtained which can in vitro transport Ca2+ simultaneously with phosphorylation of the added ADP. Kinetic advantages of succinate in providing oxidative phosphorylation as compared with NAD-dependent substrates are much more pronounced in mitochondria of the thin intestine mucosa than in other tissues, and Ca2+ transport occurred only under succinate oxidation. As compared with liver mitochondria these mitochondria are shown to be more sensitive to adrenaline and calcium action.
Databáze: OpenAIRE