| Popis: |
HVS-MEST cells, which are adherent, produced more infectious HVS at 37 degress C than at 33 degrees C. This was paralleled by the appearance of EA and LA in rounded cells and CF antigen in the cell cultures. In contrast, MLC-1 cells, which grow in suspension, produced hardly any infectious virus, but did produce more HVS S antigen than did the HVS-MEST cells. Production of S antigen in MLC-1 cells was augmented by treatment of the cells with BUDR. HVS-MEST cells could be superinfected with HVS, but MLC-1 cells were resistant to super-infection. The unpurified S antigens from both cell lines gave almost identical spectra when tested in CF against a battery of 10 owl and squirrel monkey sera. Thus, two cell lines have been described, one that produces infectious virus and S antigen, and another that produces more S antigen, but in the absence of any great amounts of infectious virus. |
