Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells

Autor:	M A, Axelsson, E M, Hansson, R, Sikut, G C, Hansson
Rok vydání:	1998
Předmět:	Mucin-3 Mucin-2 Nylons Blotting Western Mucin-1 Mucins Antibodies Monoclonal Oligosaccharides Membranes Artificial Gases Hydrofluoric Acid
Zdroj:	Glycoconjugate journal. 15(8)
ISSN:	0282-0080
Popis:	Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0c115a11d70cb4fe1bc8c8566cb84b8f https://pubmed.ncbi.nlm.nih.gov/9870350 Zobrazit plný text záznamu Full text from SpringerLink