| Popis: |
Allergeno-active protein substrate of enterobacteria (E. coli and R. rettgeri strains) was divided into 7 fractions with a different molecular weight with the aid of many-stage gel-filtration through Sephadex (G-50, G-75, G-100, G-150, G-200). A study was made of their biochemical composition, antigenic and protein spectrum, allergenic properties in the reactions of hypersensitivity of delayed type in vivo (skin tests) and in vitro (inhibition of macrophage migration). The principal part of allergenic activity was determined by components with a molecular weight of 30-150 and over 800 thousand. The majority of the antigens had a molecular weight of 30-150 thousand. A physico-chemical heterogeneity of immunologically affiliated components was noted. The allergenic substrate of the bacterial cell included a complex of molecular-nonhomogenous "strong" and "weak" protein allergens. |
