Peculiarities of ultrastructural organization and metabolism of reactive forms of oxygen and nitrogen in a cardiovascular system for permanent effects of ionizing radiation in low doses
| Autor: | I V, Horot, M M, Tkachenko |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Radioactive Fallout Radioisotopes Mice Inbred BALB C Nitric Oxide Synthase Type III Portal Vein Myocardium Primary Cell Culture Endothelial Cells Nitric Oxide Synthase Type II Apoptosis Dose-Response Relationship Radiation Radiation Dosage Radiation Tolerance Mice Oxidative Stress Chernobyl Nuclear Accident Gamma Rays Peroxynitrous Acid Animals Female Myocytes Cardiac Reactive Oxygen Species Ukraine Aorta |
| Zdroj: | Problemy radiatsiinoi medytsyny ta radiobiolohii. 22 |
| ISSN: | 2304-8336 |
| Popis: | Determination of the peculiarities of ultrastructural changes and metabolism of reactive forms of oxy gen and nitrogen in the tissues of the myocardium, aorta and portal vein of the radiosensitive BALB/c mice due long term exposure to the complex of radionuclides of Chоrnobyl fallout (ejection) and low intensity low dose γ irradiation.Experimental studies were performed on 60 mice female radiosensitive lines BALB/c with a body weight of 20-22 g, which were divided into 3 groups: I group (control) animals age 6-9 months which were born and lived their lives in Kyiv vivarium under conditions natural radioactive background; ІІ - animals age 6 months, which were born and lived in the Chornobyl exclusion zone throughout their lives; ІІІ - animals, which from 3 months of age for 6 months were constantly located in cages with flat ionizing radiation sources and exposed to external γ irradiation in a total dose of 0.43 Sv. Animals of II and III groups were kept in the vivarium of the Institute for Safety Problems of Nuclear Power Plants of the National Academy of Sciences of Ukraine (Chornobyl). Mice of the BALB/c lines are sensitive to radiation: for males LD 50/305.7 Gy, for females - 5.85 Gy.The peculiarities of structural and functional changes in the organs of the cardiovascular system (CVS) and mechanisms of the development of oxidative and nitrosatitative stress due long term exposure to the complex of radionuclides of the Chornobyl fallout (ejection) and low intensity low dose γ radiation based on the study of ultrastructural organization and metabolism of reactive oxygen species (ROS) and nitrogen (NRS) of the atri al cardiomyocytes (CMC), the aortic endothelium and portal vein of the BALB/c mice. Found that the permanent expo sure to low doses of radiation changes occur, including the dominant mechanism is to run a CMC apoptosis and endothelial cells of the aorta and portal vein. It is shown that the development of oxidative stress accompanied by increased activity of ROS metabolism that occurs due to activation of nucleotide and lipid superoxide generators. Found that under these conditions the development of nitrosative stress is due to the activation of inducible NO syn thesis enzyme iNOS and inhibit its synthesis constitutive eNOS enzyme. High levels of simultaneous generation of superoxide and NO, bindting, lead to the formation of toxic peroxynitrite, which initiates the process of apoptosis.Meta. Vyznachennia osoblyvostey̆ ul'trastrukturnykh zmin ta metabolizmu reaktyvnykh form kysniu i azotu v tka nynakh miokarda, aorty i voritnoï veny myshey̆ radiochutlyvoï liniï BALB/c za umov tryvaloï diï sukupnosti radionuklidiv chornobyl's'kogo vykydu ta nyz'kointensyvnogo γ vyprominiuvannia v nyz'kykh dozakh.Materialy i metody. Eksperymental'ni doslidzhennia provedeno na 60 myshakh samtsiakh radiochutlyvoï liniï BALB/c masoiu tila 20–22 g, iakykh bulo rozpodileno na 3 grupy. grupa I (kontrol') – tvaryny vikom 6–9 mis., iaki narody lysia i prozhyly svoie zhyttia u kyïvs'komu vivariï za umov pryrodnogo radioaktyvnogo fonu; grupa II – tvaryny vikom 6 mis., iaki narodylysia i vse zhyttia znakhodylysia v Chornobyl's'kiy̆ zoni vidchuzhennia; grupa III – tvaryny, iaki z trymisiachnogo viku uprodovzh 6 mis. postiy̆no perebuvaly u klitkakh z ploskymy dzherelamy ionizuiuchogo vyp rominiuvannia i zaznaly zovnishn'ogo γ oprominennia u sumarniy̆ dozi 0,43 Zv. Tvaryny grup II i III utrymuvalysia u vivariï Instytutu problem bezpeky atomnykh elektrostantsiy̆ NAN Ukraïny (m. Chornobyl').Rezul'taty i vysnovky. Vyiavleni osoblyvosti strukturno funktsional'nykh zmin v organakh sertsevo sudynnoï systemy ta mekhanizmiv rozvytku oksydatyvnogo i nitrozatyvnogo stresu za umov tryvaloï diï sukupnosti radionuklidiv chornobyl's'kogo vykydu ta nyz'kointensyvnogo γ vyprominiuvannia v nyz'kykh dozakh na pidstavi doslidzhennia ul'trastrukturnoï organizatsiï i metabolizmu reaktyvnykh form kysniu i azotu peredserdnykh kardiomiotsytiv, endoteliiu aorty i voritnoï veny myshey̆ liniï BALB/c. Vstanovleno, shcho za tryvaloï diï nyz'kykh doz radiatsiï vynykaiut' zminy, sered iakykh dominuiuchym ie zapusk mekhanizmiv apoptozu iak kardiomiotsytiv, tak i en dotelial'nykh klityn aorty i voritnoï veny. Pokazano, shcho rozvytok oksydatyvnogo stresu suprovodzhuiet'sia pidvyshchenniam aktyvnosti metabolizmu reaktyvnykh form kysniu, shcho vidbuvaiet'sia za rakhunok aktyvatsiï nukleotyd nogo i lipidnogo generatoriv superoksydu. Vyiavleno, shcho za tsykh umov rozvytok nitrozatyvnogo stresu vidbuvaiet' sia vnaslidok aktyvatsiï indutsybel'nogo syntezu NO fermentom iNOS ta ingibuvannia konstytutyvnogo y̆ogo synte zu fermentom eNOS. Vysoki rivni odnochasnoï generatsiï superoksydu i NO, zv'iazuiuchys', pryzvodiat' do utvorennia toksychnogo peroksynitrytu, iakyy̆ ie initsiatorom protsesu apoptozu. |
| Databáze: | OpenAIRE |
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