Antisense methods for discrimination of phenotypic properties of closely related gene products: Jun kinase family
| Autor: | F, Bost, R, McKay, N M, Dean, O, Potapova, D, Mercola |
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| Rok vydání: | 1999 |
| Předmět: |
Mice
Knockout Transplantation Heterologous JNK Mitogen-Activated Protein Kinases Gene Expression Oligonucleotides Antisense Protein-Tyrosine Kinases Thionucleotides Transfection Isoenzymes Mice Cell Transformation Neoplastic Phenotype Mitogen-Activated Protein Kinase 10 Multigene Family Liposomes Animals Mitogen-Activated Protein Kinase 9 Mitogen-Activated Protein Kinases Protein Kinases Half-Life |
| Zdroj: | Methods in enzymology. 314 |
| ISSN: | 0076-6879 |
| Popis: | Methods for the selection and characterization of antisense oligonucleotides for specifically eliminating closely related gene family members are available. High-throughput semiautomated methods using 96-well plate formats and array technology and improved assays are under active development that will streamline many steps and will likely merge. Second-generation 20-mer antisense phosphorothioate oligonucleotides containing 2'-methoxyethyl groups at the first and last 6 nucleotides with improved nuclease resistance and RNA affinity are becoming available. |
| Databáze: | OpenAIRE |
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